Perfusion double-channel micropipette probes for oxygen flux mapping with single-cell resolution

• Yang Gao,
• Bin Li,
• Riju Singhal,
• Adam Fontecchio,
• Ben Pelleg,
• Zulfiya Orynbayeva,
• Yury Gogotsi and
• Gary Friedman

Beilstein J. Nanotechnol. 2018, 9, 850–860, doi:10.3762/bjnano.9.79

• as individual cells at subcellular resolution. Keywords: double-barrel pipette; hydrodynamic confinement; perfusion; oxygen flux; single-cell metabolic analysis; Introduction Transport, production and consumption of gasses, ions, and organic molecules are fluxes that sustain life. Relatively few

• studied below using a finite element model (FEM) of the double-barrel pipette with perfusion. To validate this model, we first compare hydrodynamic confinement obtained from the model with experiments using a fluorescent dye. Later in the paper, we also show that FEM results agree qualitatively with a

• simplified analytical model. Results and Discussion Hydrodynamic confinement Consider flow within a long channel: a molecule cannot diffuse outside the channel due to the presence of hard channel walls. However, if the channel walls are missing along some length segment of the flow, the molecule may diffuse